Journal: Frontiers in Molecular Biosciences

Article Title: A prognostic nomogram for colorectal cancer based on ubiquitin-specific protease 21 expression: a retrospective cohort study

doi: 10.3389/fmolb.2026.1764916

Figure Lengend Snippet: Expression and clinical significance of USP21 in CRC based on public database analysis. (A) USP21 transcriptional expression levels across various malignant tumor tissues based on TIMER database. (B) USP21 expression levels in CRC from TCGA database. (C) USP21 expression levels in the GSE83889 dataset. (D) ROC curve for USP21 expression in diagnosing TCGA-CRC. (E) Kaplan-Meier survival curves for overall survival (OS) stratified by USP21 expression in TCGA-CRC.

Article Snippet: USP21 protein expression was assessed by streptavidin–peroxidase immunohistochemistry using a rabbit monoclonal anti-USP21 antibody (1:400, Boster, A06639-1).

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: A prognostic nomogram for colorectal cancer based on ubiquitin-specific protease 21 expression: a retrospective cohort study

doi: 10.3389/fmolb.2026.1764916

Figure Lengend Snippet: Upregulation of USP21 correlates with poor prognosis in CRC patients. (A) Representative immunohistochemical staining of USP21 in CRC tissues. (B) Kaplan–Meier survival curves for high and low USP21 expression groups.

Article Snippet: USP21 protein expression was assessed by streptavidin–peroxidase immunohistochemistry using a rabbit monoclonal anti-USP21 antibody (1:400, Boster, A06639-1).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: eLife

Article Title: Transcriptional correlates of malaria in RTS,S/AS01-vaccinated African children: a matched case–control study

doi: 10.7554/eLife.70393

Figure Lengend Snippet:

Article Snippet: Antibody , anti-IL-21, clone 3A3-N2 (mouse monoclonal) , Miltenyi Biotec , Cat# 130-120-702 , Fluorochrome: APC.

Techniques: Staining, Concentration Assay, Recombinant, Produced, Sequencing, Isolation, Gel Extraction, Software, Adhesive, Reverse Transcription, Magnetic Beads

Journal: Immunity

Article Title: Low-Avidity CD4 + T Cell Responses to SARS-CoV-2 in Unexposed Individuals and Humans with Severe COVID-19

doi: 10.1016/j.immuni.2020.11.016

Figure Lengend Snippet:

Article Snippet: IL-21-PE (REA1039) , Miltenyi Biotec , Cat#130-117-421; RRID: AB_2727941.

Techniques: Functional Assay, Recombinant, Staining, Sequencing, Gene Expression, Software

Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: Galectin 9 , REA435 , 50 , 130-124-237 , PE (APC) , Miltenyi Biotec.

Techniques: Imaging

Journal: iScience

Article Title: Oleic acid triggers metabolic rewiring of T cells poising them for T helper 9 differentiation

doi: 10.1016/j.isci.2024.109496

Figure Lengend Snippet:

Article Snippet: Anti-human IL-22; Vio® B515; Isotype Human; Clone REA466 , Miltenyi Biotec , Cat#130-108-096; RRID: AB_2652431.

Techniques: Activation Assay, Control, Isolation, Recombinant, Modification, Staining, cDNA Synthesis, Gene Expression, Software

Journal: Scientific Reports

Article Title: Combining DNMT and HDAC6 inhibitors increases anti-tumor immune signaling and decreases tumor burden in ovarian cancer

doi: 10.1038/s41598-020-60409-4

Figure Lengend Snippet: DNMT1 protein levels are decreased by combination treatment of DNMTi and HDAC6i. ( A ) Ovarian cancer cell lines were treated as in Fig. and protein was extracted at Day 7 after treatment with IFN-gamma (IFN-γ+) (to assess MHC I and PD-L1 expression, in later figures) or control (IFN-γ -). Protein was isolated and immunoblots were run for the DNMT1 protein and α-tubulin as a loading control. Immunoblot membranes were cut and probed separately for DNMT1 (about 188 kDa) and α-tubulin (50 kDa). Cropped blots are shown here, and black lines indicate where one part of the blot ends and another begins. Figure shows the entire blot images. ( B ) The TykNu cell line was treated as in ( A ) and the protein synthesis cycloheximide added to cells on Day 7 for 0, 4, and 8 hours at 10 μM as indicated on the blot. Protein was isolated and immunoblots were run for the DNMT1 protein and α-tubulin as a loading control. Immunoblot membranes were cut and probed separately for DNMT1 (about 188 kDa) and α-tubulin (50 kDa). Cropped blots are shown here, and black lines indicate where one part of the blot ends and another begins. Figure shows the entire blot images. ( C ) Stable knockdowns of the HDAC6 protein were generated in the ID8 Trp53+/+ and Trp53−/− cell lines . Protein was extracted and immunoblots were run for the DNMT1 protein with B-actin as a loading control. Immunoblot membranes were probed for DNMT1 (about 188 kDa) and α-tubulin (50 kDa). Cropped blots are shown here and black lines indicate where one part of the blot ends and another begins. Figure shows the entire blot images. ( D ) Immunoblot showing knockdown of HDAC6 protein with a-Tubulin as a loading control. Protein was extracted and immunoblots were run for the HDAC6 protein with B-actin as a loading control. Immunoblots were probed for HDAC6 (131 kDa) and tubulin (50 kDa). Cropped blots are shown here and black lines indicate where one part of the blot ends and another begins. Figure shows the entire blot images. ( E ) Ovarian cancer cell lines were treated as in Fig. and RNA was extracted at Day 7. qRT-PCR was run for DNMT1, DNMT3a, and DNMT3b and TBP was used as a reference gene. *p < 0.05 compared to Mock.

Article Snippet: The antibodies used for immunoblotting included: DNMT1 (Sigma, D4692), PD-L1 (ProSci, 4059), HDAC1 (Cell Signaling, 2062), HDAC2 (Cell Signaling, 2540), HDAC6 (Assay Biotech, C0026), acetyl-alpha Tubulin (Cell Signaling, 3971), alpha-Tubulin (Cell Signaling, 3873).

Techniques: Expressing, Control, Isolation, Western Blot, Generated, Knockdown, Quantitative RT-PCR